Description
Immobilon®-P PVDF Western Blotting Membrane, 0.45 µm – Millipore | 27 cm × 3.75 m Roll
Immobilon®-P is Millipore’s benchmark PVDF membrane for western blot protein transfer and one of the most cited blotting membranes in the scientific literature. The 0.45 µm pore size delivers high protein binding capacity and is particularly suited for standard through high molecular weight proteins. The hydrophobic PVDF matrix provides exceptional mechanical strength, enabling multiple stripping and re-probing cycles without membrane degradation or significant signal loss — an important advantage for multi-target detection from a single blot. Immobilon-P is fully compatible with wet and semi-dry transfer systems, and with all standard detection methods including enhanced chemiluminescence (ECL), alkaline phosphatase or HRP colorimetric substrates, and fluorescent secondary antibodies. Brief methanol activation is required before use. Supplied as a 27 cm × 3.75 m bulk roll for custom cutting to any blot format.
Specifications
| Parameter | Specification |
|---|---|
| Membrane Material | PVDF (polyvinylidene fluoride) |
| Pore Size | 0.45 µm |
| Protein Binding Capacity | >150 µg/cm² |
| Roll Dimensions | 27 cm × 3.75 m |
| Detection Compatibility | ECL, colorimetric, fluorescent |
| Re-probing | Yes (multiple strip and re-probe cycles) |
| Pre-wetting | Methanol activation required |
| Transfer Method Compatibility | Wet and semi-dry |
| Cat No. | IPVH00010 |
| Brand | Millipore |
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