Description
Ascentis® C8 HPLC Column, 5 µm, 15 cm × 4.6 mm – Supelco by Merck | Cat # 581424-U
The Ascentis® C8 HPLC column from Supelco by Merck is a high-performance reversed-phase column designed for analytes that require a moderately hydrophobic stationary phase. The C8 octyl bonded phase provides less retention than C18 phases, making it an ideal choice for highly non-polar compounds or methods requiring shortened run times without sacrificing selectivity.
This column stands out with a notably high carbon loading of 15% and surface coverage of 4.0 µmol/m² on a high-purity spherical silica base with 450 m²/g surface area — figures that are significantly higher than many competing C8 phases, resulting in stronger overall retention and better loading capacity for preparative or high-concentration sample workflows. The 100 Å pore size and fully porous particle platform provide efficient mass transfer and good efficiency for small molecule separations.
Endcapping minimises residual silanol interactions and improves peak symmetry for basic compounds. The column operates across pH 2–8 with a maximum temperature of 70°C and maximum pressure of 400 bar, supporting standard HPLC and LC/MS applications. Metal impurities below 5 ppm ensure compatibility with metal-sensitive analytes, and USP L7 certification enables use in pharmacopoeial analytical procedures.
Specifications
| Parameter | Specification |
|---|---|
| Phase | C8 (Octyl) |
| Particle Size | 5 µm |
| Particle Type | Fully porous, spherical |
| Matrix | High-purity silica gel |
| Carbon Loading | 15% |
| Surface Area | 450 m²/g |
| Surface Coverage | 4.0 µmol/m² |
| Pore Size | 100 Å |
| Endcapped | Yes |
| Column Dimension (L × I.D.) | 15 cm × 4.6 mm |
| Operating pH Range | 2–8 |
| Max. Temperature | ≤70 °C |
| Max. Pressure | 400 bar (5801 psi) |
| Metal Impurities | <5 ppm |
| Column Material | Stainless steel |
| Catalog Number | 581424-U |
| USP Classification | L7 |
| Separation Technique | Reversed phase |
| Compatible Techniques | HPLC, LC/MS |
| Brand | Supelco by Merck |
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